Virus isolation could be done on swabs or lung tissue. We evaluate the pathogenesis of PPR genotype IV virus after infection of two groups of goats with viral load detection in secretions and tissues, compared to two uninfected goats. 1988;52:46–52. KT participated in the design and the follow up of the study. The aim of this study is to understand infection chronology, virus circulation, and contribute to the disease early detection. One goat presented a mild dyspnea and alimentation decrease in the last days. Peste des petits ruminants (PPR) is a viral disease which primarily affects small ruminants, causing significant economic losses for the livestock industry in developing countries. Mc chesney MB, Miller CJ, Rota PA, Zhu Y, Antipa L, Lerche NW, et al. Yanagi Y, Takeda M, Ohno S. Measles virus: cellular receptors, tropism and pathogenesis. All authors read and approved the final manuscript. -. In: Rinderpest and Peste des Petits ruminants.  |  Swabs and tissues showed high charge of the virus and clinical signs dominated by pulmonary attack and digestive symptoms secondary. Both goats of group I had a 1.5 log10 value of antibody neutralizing titer at 9 dpi by VNT. 2016;11:1–18. The cycling conditions were as follows: Reverse transcription 45 °C for 10 min; reverse transcription inactivation and DNA polymerase activation 95 °C for 2 min; followed by 40 cycles of amplification (95 °C for 5 s and 60 °C for 20 s). Couacy-Hymann E, Bodjo SC, Danho T, Koffi MY, Libeau G, Diallo A. For each individual goat, a severity score was calculated. Virus penetrates the retropharyngeal mucosa, sets up a viremia and specifically damages the alimentary, lymphoid and respiratory system. PPRV infection was experimentally induced in 4 six-month-old goats by intra-nasal and intravenous route of cell virus suspension and from infectious mashed tissue. Antibody positive and negative cut-off values were calculated as recommended by manufacturer with the sera being negative, if a percentage of inhibition was < 30%, and positive if a percentage of inhibition was > 30%. Vaccine. In spite of the importance of PPR as the priority disease for eradication, very few studies have been dedicated to describe infection kinetic in the animal body [20, 25,26,27,28]. Truong T, Boshra H, Embury-Hyatt C, Nfon C, Gerdts V, Tikoo S, Babiuk LA, Kara P, Chetty T, Mather A, Wallace DB, Babiuk S. PLoS One. For both groups I and II lacrimal PPRV genome excretion was detected with similar value from D3, nasal PPRV genome excretion from D6, rectal PPRV genome excretion at D6. Results presented are average temperature of four goats infected with cell virus suspension and infectious mashed tissue. ME participated in the design of the study, data analysis, interpretations and manuscript drafting. All of the immune cells (lymphocytes, macrophages, reticular cells) can be a target for virus multiplication. 2013;8:e55830. Briefly, 25 μl of sera samples were added to 96 and incubated for 1 h at 37 °C. Google Scholar. Special Issue Information. Following inoculation with PPRV, four goats of the two groups showed typical PPR signs from 4 dpi, i.e. Peste des petits ruminants (PPR) is a viral disease of major economic importance on small ruminants. Animals were partially exsanguinated by jugular catheterization. Peste des petits ruminants (PPR) represents one of the most economically important disease and imposes a significant constraint on small ruminants [1, 2] and it was considered to be an emerging viral disease of small ruminants caused by a Morbillivirus. Fakri FZ, Elhajjam A, Bamouh Z, Jazouli M, Boumart Z, Tadlaoui K, et al. volume 15, Article number: 452 (2019) This book offers a timely and comprehensive review of essential research on Peste des Petits Ruminants Virus (PPRV), ranging from its historical distribution, molecular epidemiology, genome structure, viral proteins, immunity, viral pathogenesis, clinical and molecular diagnosis to advances in vaccine developments and future challenges. Get the latest research from NIH: https://www.nih.gov/coronavirus. London, Burlington, San Diego: Academic Press; 2006. J Gen Virol. Etiology The consent was obtained from MCI Santé Animale breeding farm to use the animals in the study. If severe clinical symptoms were observed and the cumulative clinical score arrived at a value between 15 and 18, goats were euthanized by overdose anesthesia through intravenous xylazine and intramuscular ketamine injections followed by exsanguination [34, 35]. Infected cells and supernatant were harvested and frozen at − 80 °C for subsequent experiments. http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/, https://doi.org/10.1186/s12917-019-2164-6, Clinical pathology, physiology and immunology. Arch Virol. Please enable it to take advantage of the complete set of features! 2004;51:153–9. The clinical signs were observed and goats were euthanized at predetermined clinical score level for post-mortem examinations and PPRV detection by RT-PCR. -, Jones BA, Rich KM, Mariner JC, Anderson J, Jeggo M, Thevasagayam S, et al. Molecular Biology and Pathogenesis of Peste des Petits Ruminants Virus. The datasets used and/or analyzed during the current study available from the corresponding author on reasonable request. signs and lesions observed on goats. Esolen L, Ward B, Moench T, Griffin D. Infection of monocytes during measles. Hammouchi M, Loutfi C, Sebbar G, Touil N, Chaffai N, Batten C, et al. OIE.  |  Rectal temperatures were measured 3 days prior to experimental infection with PPRV (MOR15), and following infection every day until 9 dpi. VNT test was done as described in the OIE Terrestrial Manual [13]. Furthermore, PPR is … The viral stock was passaged 8 times in Vero (African green monkey kidney cells, ATCC No.CCL-81). PLoS One. NS data analysis and critical review of the manuscript. Comp Immunol Microbiol Infect Dis. Although PPRV-infected animals were known to exhibit clinical signs similar to rinderpest [1], it was not until the late 1970’s that PPR pathogenesis was evaluated in … NIH Medawar and Medawar Results presented are average temperature of four goats infected with cell virus suspension and infectious mashed tissue, signs and lesions observed on goats. The authors are experts in the field and provide an up-to-date and comprehensive review covering all aspects of the disease. Onderstepoort J Vet Res. Wernike K, Eschbaumer M, Breithaupt A, Maltzan J, Wiesner H, Beer M, Hoffmann B. Vet Microbiol. Epub 2014 May 13. Early events following experimental infection with Peste-des-Petits ruminants virus suggest immune cell targeting. Peste des petits ruminants. COVID-19 is an emerging, rapidly evolving situation. -, Albina E, Kwiatek O, Minet C, Lancelot R, Servan de Almeida R, Libeau G. Peste des petits ruminants, the next eradicated animal disease? Vet Microbiol. Goats were fed a complete balanced diet and water ad libitum and housed in animal boxes (Biosecurity level 3 containment) at MCI Santé Animale. INTRODUCTION In vitro cell culture system is a useful alternative to animal based research including drug effect study or pathogenesis of intracellular pathogen particularly virus, chlamydia and rickettsia. Despite the hyperthermia, very low viraemia is detected by PCR. Correspondence to Conclusion: Google Scholar. Early detection of viral excretion from experimentally infected goats with peste-des-petits ruminants virus. Results: Lung is the preferred source of virus among all other tissues for virus detection and isolation. Privacy We listed five criteria by significance: 1) Mortality, 2); Clinical scoring; 3) Viral load in post mortem tissue; 4) Hyperthermia duration; 5) Viral load in swabs (number of positive and cycle threshold (Ct) values).

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